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| 09.09.2016 | 18:00 | 1. FC Slovácko | - | FC Fastav Zlín | 1:3 (1:0) | |
| 10.09.2016 | 15:00 | AC Sparta Praha | - | FK Mladá Boleslav | 2:2 (1:1) | |
| 17:00 | MFK Karviná | - | Vysočina Jihlava | 3:0 (2:0) | ||
| 17:00 | FC Hradec Králové | - | 1. FK Příbram | 2:0 (0:0) | ||
| 20:15 | Slovan Liberec | - | Viktoria Plzeň | 1:2 (0:1) | ||
| 11.09.2016 | 15:00 | Bohemians Praha 1905 | - | Zbrojovka Brno | 3:0 (3:0) | |
| 17:00 | FK Jablonec | - | FK Dukla Praha | 1:1 (0:1) | ||
| 18:15 | FK Teplice | - | Slavia Praha | 2:2 (1:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Fastav Zlín | 6 | 5 | 1 | 0 | 14:4 | 10 | 16 |
| 2 |
|
AC Sparta Praha | 6 | 4 | 2 | 0 | 12:4 | 8 | 14 |
| 3 |
|
FK Mladá Boleslav | 6 | 4 | 1 | 1 | 11:5 | 6 | 13 |
| 4 |
|
Viktoria Plzeň | 6 | 4 | 1 | 1 | 11:6 | 5 | 13 |
| 5 |
|
MFK Karviná | 6 | 3 | 1 | 2 | 11:11 | 0 | 10 |
| 6 |
|
Slavia Praha | 6 | 2 | 3 | 1 | 13:9 | 4 | 9 |
| 7 |
|
FK Teplice | 6 | 2 | 3 | 1 | 6:4 | 2 | 9 |
| 8 |
|
FC Hradec Králové | 6 | 3 | 0 | 3 | 7:7 | 0 | 9 |
| 9 |
|
FK Jablonec | 6 | 2 | 2 | 2 | 10:12 | -2 | 8 |
| 10 |
|
Bohemians Praha 1905 | 6 | 2 | 1 | 3 | 6:8 | -2 | 7 |
| 11 |
|
Zbrojovka Brno | 6 | 1 | 3 | 2 | 3:8 | -5 | 6 |
| 12 |
|
FK Dukla Praha | 6 | 1 | 2 | 3 | 7:8 | -1 | 5 |
| 13 |
|
1. FC Slovácko | 6 | 1 | 2 | 3 | 9:11 | -2 | 5 |
| 14 |
|
Slovan Liberec | 6 | 1 | 2 | 3 | 4:8 | -4 | 5 |
| 15 |
|
Vysočina Jihlava | 6 | 0 | 2 | 4 | 3:10 | -7 | 2 |
| 16 |
|
1. FK Příbram | 6 | 0 | 0 | 6 | 0:12 | -12 | 0 |
| Champions League Quali. | |
| Europa League Quali. | |
| Europa League | |
| Relégation |
