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| 17.05.2009 | 17:00 | 1. FC Brno | - | FK Teplice | 1:1 (0:0) | |
| 17:00 | SK Kladno | - | Slovan Liberec | 0:0 (0:0) | ||
| 17:00 | FK Baumit Jablonec | - | 1. FK Příbram | 4:0 (1:0) | ||
| 17:00 | FK Mladá Boleslav | - | Viktoria Plzeň | 1:1 (0:0) | ||
| 17:00 | Dynamo České Budějovice | - | Baník Ostrava | 3:2 (1:1) | ||
| 17:00 | Sigma Olomouc | - | Tescoma Zlín | 1:1 (0:0) | ||
| 20:00 | AC Sparta Praha | - | Bohemians Praha | 1:0 (0:0) | ||
| 18.05.2009 | 17:15 | Viktoria Žižkov | - | Slavia Praha | 1:3 (0:3) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 28 | 17 | 7 | 4 | 54:23 | 31 | 58 |
| 2 |
|
AC Sparta Praha | 28 | 14 | 8 | 6 | 42:23 | 19 | 50 |
| 3 |
|
Slovan Liberec | 28 | 13 | 9 | 6 | 37:26 | 11 | 48 |
| 4 |
|
FK Mladá Boleslav | 28 | 12 | 10 | 6 | 38:33 | 5 | 46 |
| 5 |
|
Sigma Olomouc | 28 | 12 | 8 | 8 | 35:35 | 0 | 44 |
| 6 |
|
FK Teplice | 28 | 12 | 7 | 9 | 33:21 | 12 | 43 |
| 7 |
|
FK Baumit Jablonec | 28 | 13 | 4 | 11 | 35:33 | 2 | 43 |
| 8 |
|
Viktoria Plzeň | 28 | 9 | 10 | 9 | 42:38 | 4 | 37 |
| 9 |
|
Baník Ostrava | 28 | 10 | 5 | 13 | 36:35 | 1 | 35 |
| 10 |
|
1. FC Brno | 28 | 9 | 8 | 11 | 30:32 | -2 | 35 |
| 11 |
|
Dynamo České Budějovice | 28 | 6 | 15 | 7 | 27:35 | -8 | 33 |
| 12 |
|
Bohemians Praha | 28 | 9 | 4 | 15 | 31:42 | -11 | 31 |
| 13 |
|
SK Kladno | 28 | 8 | 7 | 13 | 21:39 | -18 | 31 |
| 14 |
|
1. FK Příbram | 28 | 7 | 7 | 14 | 25:39 | -14 | 28 |
| 15 |
|
Tescoma Zlín | 28 | 6 | 8 | 14 | 23:42 | -19 | 26 |
| 16 |
|
Viktoria Žižkov | 28 | 5 | 7 | 16 | 27:40 | -13 | 22 |
| Champions League Quali. | |
| Europa League | |
| Relégation |
