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| 30.04.2010 | 18:20 | 1. FC Slovácko | - | FK Mladá Boleslav | 1:0 (0:0) | |
| 01.05.2010 | 16:00 | Slovan Liberec | - | Bohemians 1905 | 1:1 (0:0) | |
| 18:20 | 1. FC Brno | - | Viktoria Plzeň | 2:2 (1:1) | ||
| 20:00 | FK Teplice | - | SK Kladno | 5:0 (3:0) | ||
| 02.05.2010 | 16:00 | Bohemians Praha | - | FK Baumit Jablonec | 0:4 (0:3) | |
| 16:00 | Dynamo České Budějovice | - | Sigma Olomouc | 2:1 (1:0) | ||
| 16:00 | Slavia Praha | - | 1. FK Příbram | 3:0 (0:0) | ||
| 18:25 | Baník Ostrava | - | AC Sparta Praha | 1:1 (1:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
AC Sparta Praha | 27 | 14 | 13 | 0 | 35:12 | 23 | 55 |
| 2 |
|
Baník Ostrava | 27 | 16 | 7 | 4 | 41:22 | 19 | 55 |
| 3 |
|
FK Baumit Jablonec | 27 | 16 | 6 | 5 | 37:24 | 13 | 54 |
| 4 |
|
FK Teplice | 27 | 15 | 8 | 4 | 43:23 | 20 | 53 |
| 5 |
|
Viktoria Plzeň | 27 | 11 | 10 | 6 | 34:27 | 7 | 43 |
| 6 |
|
Sigma Olomouc | 27 | 12 | 4 | 11 | 43:35 | 8 | 40 |
| 7 |
|
FK Mladá Boleslav | 27 | 11 | 5 | 11 | 45:37 | 8 | 38 |
| 8 |
|
Slavia Praha | 27 | 10 | 8 | 9 | 34:28 | 6 | 38 |
| 9 |
|
Slovan Liberec | 27 | 9 | 6 | 12 | 30:34 | -4 | 33 |
| 10 |
|
1. FK Příbram | 27 | 9 | 5 | 13 | 31:38 | -7 | 32 |
| 11 |
|
1. FC Brno | 27 | 8 | 7 | 12 | 27:37 | -10 | 31 |
| 12 |
|
Bohemians 1905 | 27 | 7 | 9 | 11 | 19:26 | -7 | 30 |
| 13 |
|
Dynamo České Budějovice | 27 | 6 | 10 | 11 | 22:30 | -8 | 28 |
| 14 |
|
1. FC Slovácko | 27 | 7 | 5 | 15 | 24:35 | -11 | 26 |
| 15 |
|
SK Kladno | 27 | 7 | 3 | 17 | 23:45 | -22 | 24 |
| 16 |
|
Bohemians Praha | 27 | 4 | 2 | 21 | 25:60 | -35 | -6 |
| Champions League Quali. | |
| Europa League | |
| Relégation |
