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| 31.03.2000 | 17:00 | FC Boby-sport Brno | - | Dynamo České Budějovice | 2:0 (0:0) | |
| 02.04.2000 | 16:30 | Baník Ostrava | - | FK Teplice | 2:2 (0:0) | |
| 16:30 | Chmel Blsany | - | Viktoria Žižkov | 0:2 (0:0) | ||
| 16:30 | SK Hradec Králové | - | FK Jablonec 97 | 0:0 (0:0) | ||
| 16:30 | Sigma Olomouc | - | FC Dukla Pribram | 1:0 (0:0) | ||
| 16:30 | Slavia Praha | - | FC Petra Drnovice | 2:1 (1:1) | ||
| 16:30 | Slovan Liberec | - | SFC Opava | 1:0 (0:0) | ||
| 19:00 | CU Bohemians Praha | - | AC Sparta Praha | 0:1 (0:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 23 | 19 | 4 | 0 | 43:11 | 32 | 61 |
| 2 |
|
AC Sparta Praha | 23 | 18 | 3 | 2 | 60:19 | 41 | 57 |
| 3 |
|
FC Petra Drnovice | 23 | 12 | 4 | 7 | 31:21 | 10 | 40 |
| 4 |
|
FK Teplice | 23 | 8 | 7 | 8 | 33:33 | 0 | 31 |
| 5 |
|
CU Bohemians Praha | 23 | 8 | 7 | 8 | 19:20 | -1 | 31 |
| 6 |
|
Chmel Blsany | 23 | 9 | 3 | 11 | 22:36 | -14 | 30 |
| 7 |
|
FC Boby-sport Brno | 23 | 8 | 5 | 10 | 25:28 | -3 | 29 |
| 8 |
|
Slovan Liberec | 23 | 6 | 10 | 7 | 14:16 | -2 | 28 |
| 9 |
|
Baník Ostrava | 23 | 6 | 9 | 8 | 34:36 | -2 | 27 |
| 10 |
|
FC Dukla Pribram | 23 | 7 | 6 | 10 | 23:31 | -8 | 27 |
| 11 |
|
Dynamo České Budějovice | 23 | 8 | 3 | 12 | 26:35 | -9 | 27 |
| 12 |
|
Sigma Olomouc | 23 | 5 | 11 | 7 | 25:30 | -5 | 26 |
| 13 |
|
Viktoria Žižkov | 23 | 6 | 7 | 10 | 30:37 | -7 | 25 |
| 14 |
|
SFC Opava | 23 | 5 | 8 | 10 | 26:29 | -3 | 23 |
| 15 |
|
SK Hradec Králové | 23 | 3 | 10 | 10 | 17:28 | -11 | 19 |
| 16 |
|
FK Jablonec 97 | 23 | 4 | 7 | 12 | 17:35 | -18 | 19 |
