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| 16.01.2009 | 20:00 | FC Dordrecht | - | TOP Oss | 2:0 (1:0) | |
| 20:00 | FC Eindhoven | - | Telstar | 1:4 (0:2) | ||
| 20:00 | Fortuna Sittard | - | Helmond Sport | 3:1 (1:0) | ||
| 20:00 | HFC Haarlem | - | SC Cambuur | 0:1 (0:0) | ||
| 20:00 | BV Veendam | - | MVV | 1:0 (1:0) | ||
| 20:00 | FC Omniworld | - | FC Emmen | 1:0 (0:0) | ||
| 20:00 | AGOVV | - | RKC Waalwijk | 2:2 (0:2) | ||
| 20:00 | VVV-Venlo | - | RBC Roosendaal | 3:1 (3:0) | ||
| 20:00 | FC Den Bosch | - | SBV Excelsior | 0:0 (0:0) | ||
| 18.01.2009 | 12:30 | Go Ahead Eagles | - | FC Zwolle | 0:1 (0:1) |
| » Classement | équipe recevante | à l'extérieur | Premier tour | Second tour |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
VVV-Venlo | 22 | 16 | 3 | 3 | 53:22 | 31 | 51 |
| 2 |
|
RKC Waalwijk | 22 | 9 | 12 | 1 | 35:21 | 14 | 39 |
| 3 |
|
SC Cambuur | 22 | 11 | 5 | 6 | 41:32 | 9 | 38 |
| 4 |
|
SBV Excelsior | 22 | 10 | 7 | 5 | 33:21 | 12 | 37 |
| 5 |
|
MVV | 22 | 9 | 7 | 6 | 34:24 | 10 | 34 |
| 6 |
|
FC Zwolle | 22 | 9 | 7 | 6 | 36:32 | 4 | 34 |
| 7 |
|
FC Den Bosch | 22 | 8 | 8 | 6 | 37:28 | 9 | 32 |
| 8 |
|
Helmond Sport | 22 | 8 | 8 | 6 | 33:29 | 4 | 32 |
| 9 |
|
HFC Haarlem | 22 | 10 | 2 | 10 | 28:34 | -6 | 32 |
| 10 |
|
Go Ahead Eagles | 22 | 7 | 8 | 7 | 21:27 | -6 | 29 |
| 11 |
|
AGOVV | 22 | 8 | 4 | 10 | 33:40 | -7 | 28 |
| 12 |
|
Fortuna Sittard | 22 | 7 | 6 | 9 | 26:36 | -10 | 27 |
| 13 |
|
Telstar | 22 | 6 | 8 | 8 | 32:34 | -2 | 26 |
| 14 |
|
RBC Roosendaal | 22 | 6 | 7 | 9 | 27:29 | -2 | 25 |
| 15 |
|
BV Veendam | 22 | 6 | 8 | 8 | 27:35 | -8 | 23 |
| 16 |
|
TOP Oss | 22 | 6 | 5 | 11 | 25:36 | -11 | 23 |
| 17 |
|
FC Eindhoven | 22 | 5 | 10 | 7 | 37:43 | -6 | 22 |
| 18 |
|
FC Emmen | 22 | 5 | 6 | 11 | 27:43 | -16 | 21 |
| 19 |
|
FC Dordrecht | 22 | 4 | 6 | 12 | 21:26 | -5 | 18 |
| 20 |
|
FC Omniworld | 22 | 4 | 5 | 13 | 23:37 | -14 | 17 |
| Promotion | |
| Play-off | |
| Play-off | |
| Play-off | |
| Play-off |
