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| 08.03.2010 | 20:00 | FC Zwolle | - | AGOVV | 2:2 (1:0) | |
| 20:00 | Helmond Sport | - | Fortuna Sittard | 0:0 (0:0) | ||
| 20:00 | De Graafschap | - | SC Cambuur | 3:0 (1:0) | ||
| 20:00 | Telstar | - | SBV Excelsior | 1:2 (0:1) | ||
| 20:00 | MVV | - | FC Eindhoven | 2:2 (2:0) | ||
| 20:00 | FC Omniworld | - | Go Ahead Eagles | 2:0 (2:0) | ||
| 20:00 | RBC Roosendaal | - | FC Emmen | 1:1 (0:0) | ||
| 20:00 | FC Dordrecht | - | FC Oss | 0:1 (0:1) | ||
| 22.03.2010 | 20:00 | FC Den Bosch | - | FC Volendam | 3:0 (1:0) |
| » Classement | équipe recevante | à l'extérieur | Premier tour | Second tour |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
De Graafschap | 29 | 19 | 6 | 4 | 59:23 | 36 | 63 |
| 2 |
|
SC Cambuur | 29 | 16 | 8 | 5 | 66:44 | 22 | 56 |
| 3 |
|
Go Ahead Eagles | 29 | 15 | 8 | 6 | 45:24 | 21 | 53 |
| 4 |
|
FC Zwolle | 30 | 15 | 7 | 8 | 46:34 | 12 | 52 |
| 5 |
|
SBV Excelsior | 29 | 15 | 5 | 9 | 57:41 | 16 | 50 |
| 6 |
|
AGOVV | 30 | 14 | 9 | 7 | 56:43 | 13 | 49 |
| 7 |
|
FC Den Bosch | 30 | 12 | 10 | 8 | 56:42 | 14 | 46 |
| 8 |
|
Helmond Sport | 29 | 13 | 6 | 10 | 56:45 | 11 | 45 |
| 9 |
|
BV Veendam | 29 | 11 | 8 | 10 | 46:39 | 7 | 40 |
| 10 |
|
RBC Roosendaal | 30 | 10 | 9 | 11 | 38:44 | -6 | 39 |
| 11 |
|
FC Dordrecht | 29 | 11 | 3 | 15 | 48:48 | 0 | 36 |
| 12 |
|
FC Eindhoven | 29 | 10 | 6 | 13 | 52:63 | -11 | 36 |
| 13 |
|
MVV | 30 | 10 | 7 | 13 | 48:59 | -11 | 36 |
| 14 |
|
FC Omniworld | 29 | 10 | 5 | 14 | 33:48 | -15 | 35 |
| 15 |
|
FC Emmen | 30 | 8 | 6 | 16 | 40:66 | -26 | 30 |
| 16 |
|
FC Volendam | 29 | 6 | 11 | 12 | 49:62 | -13 | 29 |
| 17 |
|
Fortuna Sittard | 30 | 6 | 8 | 16 | 26:47 | -21 | 26 |
| 18 |
|
Telstar | 29 | 6 | 5 | 18 | 36:54 | -18 | 23 |
| 19 |
|
FC Oss | 29 | 4 | 9 | 16 | 36:67 | -31 | 21 |
| 20 |
|
HFC Haarlem | 0 | 0 | 0 | 0 | 0:0 | 0 | 0 |
| Promotion | |
| Play-off | |
| Play-off |
