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| 18.09.2009 | RBC Roosendaal | - | HFC Haarlem | ann. | ||
| 20:00 | Helmond Sport | - | FC Eindhoven | 2:2 (1:1) | ||
| 20:00 | De Graafschap | - | Fortuna Sittard | 2:0 (0:0) | ||
| 20:00 | BV Veendam | - | FC Volendam | 4:0 (4:0) | ||
| 20:00 | FC Omniworld | - | AGOVV | 1:2 (1:1) | ||
| 20:00 | FC Dordrecht | - | SC Cambuur | 2:3 (1:1) | ||
| 20:00 | MVV | - | SBV Excelsior | 1:1 (0:0) | ||
| 20:00 | Telstar | - | FC Emmen | 4:1 (2:0) | ||
| 20.09.2009 | 12:30 | FC Zwolle | - | Go Ahead Eagles | 1:0 (0:0) | |
| 14:30 | FC Den Bosch | - | FC Oss | 3:3 (2:0) |
| » Classement | équipe recevante | à l'extérieur | Premier tour | Second tour |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Go Ahead Eagles | 8 | 6 | 1 | 1 | 13:4 | 9 | 19 |
| 2 |
|
De Graafschap | 8 | 5 | 2 | 1 | 18:5 | 13 | 17 |
| 3 |
|
FC Dordrecht | 8 | 5 | 1 | 2 | 21:9 | 12 | 16 |
| 4 |
|
SC Cambuur | 7 | 4 | 3 | 0 | 17:11 | 6 | 15 |
| 5 |
|
Helmond Sport | 7 | 3 | 3 | 1 | 16:9 | 7 | 12 |
| 6 |
|
AGOVV | 7 | 4 | 1 | 2 | 12:9 | 3 | 11 |
| 7 |
|
BV Veendam | 7 | 3 | 2 | 2 | 14:9 | 5 | 10 |
| 8 |
|
FC Den Bosch | 8 | 2 | 4 | 2 | 19:15 | 4 | 10 |
| 9 |
|
Telstar | 8 | 2 | 4 | 2 | 14:12 | 2 | 10 |
| 10 |
|
RBC Roosendaal | 7 | 3 | 1 | 3 | 9:8 | 1 | 10 |
| 11 |
|
SBV Excelsior | 8 | 2 | 4 | 2 | 14:14 | 0 | 10 |
| 12 |
|
FC Zwolle | 8 | 2 | 3 | 3 | 10:11 | -1 | 9 |
| 13 |
|
FC Volendam | 8 | 2 | 3 | 3 | 9:15 | -6 | 9 |
| 14 |
|
FC Omniworld | 8 | 2 | 1 | 5 | 9:15 | -6 | 7 |
| 15 |
|
MVV | 7 | 2 | 2 | 3 | 10:17 | -7 | 7 |
| 16 |
|
FC Eindhoven | 8 | 1 | 4 | 3 | 14:25 | -11 | 7 |
| 17 |
|
FC Oss | 8 | 1 | 3 | 4 | 9:16 | -7 | 6 |
| 18 |
|
FC Emmen | 7 | 0 | 2 | 5 | 3:14 | -11 | 2 |
| 19 |
|
Fortuna Sittard | 7 | 0 | 2 | 5 | 4:17 | -13 | 2 |
| 20 |
|
HFC Haarlem | 0 | 0 | 0 | 0 | 0:0 | 0 | 0 |
| Promotion | |
| Play-off | |
| Play-off |
